The CLA has said the second phase of the High Speed Two (HS2) rail line from the Midlands to Leeds and Manchester will blight many rural businesses without providing fair compensation for compulsory purchase.
The Association said the announcement gives no confidence to CLA members whose homes and businesses are close to the new route and who would suffer decades of uncertainty while the scheme is planned and built.
CLA President Harry Cotterell said: "The Government has rushed through today's route announcement before two major consultations on phase one have finished, showing contempt for rural land and business owners.
"The current compulsory purchase system treats landowners badly while helping to deliver profit to commercial operators."
Mr Cotterell said while the Extreme Hardship Scheme currently on offer in phase one was restrictive and hard to access, there would be nothing at all for owners affected by phase two until a new scheme is consulted upon.
He said: "The Government must review compulsory purchase legislation now rather than perceiving it as too complex.
"The CLA has published Fair Play: CLA vision for reform of the compulsory purchase system, a policy report which sets out a framework for root-and-branch reform of the compulsory purchase system.
"The Government must recognise the difficult position of rural landowners blighted by the HS2 route."